English to Russian Science Chemistry; Chem Sci/Eng

In a series of experiments aimed at optimizing the conditions for preparing a protein conjugate, we reacted trastuzumab (as the antibody moiety) with the polypeptide represented by SEQ ID NO:97 (Angiopep 2 (An2)). We used two different ""conjugation chemistries"", one of which employed the linker SATA.

In this conjugation, the succinimidyl ester within the linker SATA chemically reacts with a primary amine in the polypeptide. Generally, with copper-free click chemistry, two approaches can be used.

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Спасибо!