English to Greek Science Biology (-tech,-chem,micro-)

With new transposition tools called transposomes, many barriers to the insertion of transposons into genomic DNA are removed. Transposomes may be considered as stable synaptic complexes. In the absence of magnesium ions, transposomes are catalytically inactive. However, the transposome system eliminates the host barrier for in vivo transposition. If the transposome is electroporated into a bacterial cell, the transposon can be integrated directly into genomic (or episomal) DNA. In addition, there are many possible variations in the “payload” part of the transposon, and several are discussed in the chapter that may be useful for identifying virulence genes in bacteria. These include alkaline phosphatase translational fusion transposons and sets of transposons tagged with short, individually identifiable tags (STM). As more varieties of transposome are constructed and more species are targeted, there will be new discoveries of genes involved in host-pathogen interactions.

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